An Analysis of the Effect of PEG on Physical Properties of Cationic Liposomes Intended for Gene Therapy

An Analysis of the Effect of PEG on Physical Properties of Cationic Liposomes Intended for Gene Therapy

Student: Marshall Brooks
Department: Pharmaceutical Sciences
Advisor:

Abstract

Figure 1: Interaction of cationic liposomes with U-87 MG cells.
Figure 1: Interaction of cationic liposomes with U-87 MG cells.

The goal of this study is to determine whether PEGylated cationic liposomes (nanodelivery system) are more avidly taken up by brain cancer cells (U-87 MG) compared to non-PEGylated cationic lipsomes (nanodelivery system). Once determined we will use the GFP plasmid (pEGFPC-1) to determine if our physical characterization results correlate with transfection efficiency measurements. The long range objective is to replace the reporter plasmid with a more clinically relevant variety.

 

Figure 2: Interaction of PEGylated cationic liposomes with U87-MG
Figure 2: Interaction of PEGylated cationic liposomes with U87-MG

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